Possible mechanism of polyspermy block in human oocytes observed by time-lapse cinematography
نویسندگان
چکیده
منابع مشابه
Preliminary observations on polar body extrusion and pronuclear formation in human oocytes using time-lapse video cinematography.
In this study, we have used time-lapse video cinematography to study fertilization in 50 human oocytes that had undergone intracytoplasmic sperm injection (ICSI). Time-lapse recording commenced shortly after ICSI and proceeded for 17-20 h. Oocytes were cultured in an environmental chamber which was maintained under standard culture conditions. Overall, 38 oocytes (76%) were fertilized normally,...
متن کاملA glass tissue culture chamber for use in time-lapse cinematography.
Numerous tissue culture chambers have been devised with the object of providing the optical requirements necessary for microscopic observation of the culture, and some of these incorporate a means of perfusion to facilitate changing medium, or to introduce various agents during the period of observation (Carrel, 1931 ; de Haan, 1931, 1938; Lindbergh, 1939; Parker, 1950). A few have been designe...
متن کاملStudies of the mechanism of the electrical polyspermy block using voltage clamp during cross-species fertilization
Prevention of polyspermic fertilization in sea urchins (Jaffe, 1976, Nature (Lond.). 261:68-71) and the worm Urechis (Gould-Somero, Jaffe, and Holland, 1979, J. Cell Biol. 82:426-440) involves an electrically mediated fast block. The fertilizing sperm causes a positive shift in the egg's membrane potential; this fertilization potential prevents additional sperm entries. Since in Urechis the egg...
متن کاملEvaluation of human embryo development in in vitro fertilization- and intracytoplasmic sperm injection-fertilized oocytes: A time-lapse study
OBJECTIVE We investigated whether the insemination method (in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]) affected morphokinetic events and abnormal cleavage events in embryonic development. METHODS A total of 1,830 normal fertilized embryos were obtained from 272 IVF and ICSI cycles that underwent ovum retrieval culture using a time-lapse system (Embryoscope) from J...
متن کاملsuppression of coke formation in thermal cracking by coke inhibitors
the main purpose of this research was to:1.develop a coking model for thermal cracking of naphtha.2.study coke inhibition methods using different coke inhibitors.developing a coking model in naphtha cracking reactors requires a suitable model of the thermal cracking reactor based on a reliable kinetic model.to obtain reliable results all these models shall be solved simultaneously.for this pu...
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 2012
ISSN: 1058-0468,1573-7330
DOI: 10.1007/s10815-012-9815-x